Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression.

نویسندگان

  • Adam S Feldman
  • Jacqueline Banyard
  • Chin-Lee Wu
  • W Scott McDougal
  • Bruce R Zetter
چکیده

PURPOSE Using proteomic techniques, we sought to identify novel protein biomarkers in tissue and urine from patients with transitional cell carcinoma (TCC). EXPERIMENTAL DESIGN Urinary and tissue proteomes were analyzed and differentially expressed proteins were identified by mass spectrometry. One of the proteins, cystatin B, was further analyzed in TCC tissue by immunohistochemistry and in urine by semiquantitative Western blot analysis. RESULTS Cystatin B tissue staining intensity significantly increased concordantly with TCC grade (P = 0.0008). Elevated urinary cystatin B levels correlated with increasing tumor grade (P = 0.062) and stage (P = 0.0047). Patients with elevated levels of cystatin B had a shorter mean +/- SE time to disease recurrence (12 +/- 1.82 months) compared with patients who had low levels (28.8 +/- 2.26 months; P = 0.0047). Similarly, patients with elevated cystatin B levels had a shorter time to grade/stage progression compared with patients with low urinary cystatin B (P = 0.0007). By multivariate Cox regression analysis, an elevated cystatin B level was the most significant variable predicting disease recurrence (hazard ratio, 3.8; 95% confidence interval, 1.5-9.5; P = 0.0049) and grade/stage progression (hazard ratio, 10.4; 95% confidence interval, 1.6-201.5; P = 0.0104). CONCLUSIONS Cystatin B is elevated in tissue and urine of bladder cancer patients. Cystatin B urine levels are positively correlated with tumor grade, stage, and shorter time to disease recurrence and progression. Consequently, cystatin B may be useful as a novel predictive biomarker in TCC of the bladder.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A microRNA biomarker panel for the non-invasive detection of bladder cancer

The development of accurate, non-invasive urinary assays for bladder cancer would greatly facilitate the detection and management of a disease that has a high rate of recurrence and progression. In this study, we employed a discovery and validation strategy to identify microRNA signatures that can perform as a non-invasive bladder cancer diagnostic assay. Expression profiling of 754 human micro...

متن کامل

A Rare Urinary Bladder Fibrosarcoma with a Cystolith in a Dog

Case description: This report describes the simultaneous occurrence of fibrosarcoma and a urolith in the urinary bladder of a 12-year-old female terrier dog that was confirmed by pathology after excisional surgery. Clinical findings: The dog had some clinical signs such as frequent urination, hematuria and dysuria with an...

متن کامل

Diagnostic and Prognostic Factors in Non-Muscle-Invasive Bladder Cancer and Their Influence on Treatment and Outcomes

Objectives: This paper explores the diagnostic and staging procedures available for bladder cancer, the prognostic factors for recurrence and progression of non-muscle-invasive tumours, and the indications for intravesical therapy and cystectomy in patients with non-muscle-invasive, recurrent disease. Methods: The authors reviewed the literature on diagnosis of bladder cancer, prognostic factor...

متن کامل

Assessment of Serum Cystatin C Levels in Women with Breast Cancer Compared to the Control Group

Introduction: Breast cancer is the most common type of cancer in Iranian women, detected in 70% of cases when the disease has reached an advanced stage. The discovery of new diagnostic biomarkers is essential for the early detection of the disease. Cystatin C is a member of the cystatin family and a non-glycosylated, cysteine ​​protease inhibitor that is also used as a functional marker of the ...

متن کامل

Multi-institutional validation of the predictive value of Ki-67 labeling index in patients with urinary bladder cancer.

Several small single-center studies have reported a prognostic role for Ki-67 labeling index in advanced urothelial carcinoma of the urinary bladder. To investigate whether Ki-67 was a useful biomarker of oncological outcome after radical cystectomy for urothelial carcinoma, we assessed its expression in tumor tissue from 713 patients treated with radical cystectomy and bilateral lymphadenectom...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical cancer research : an official journal of the American Association for Cancer Research

دوره 15 3  شماره 

صفحات  -

تاریخ انتشار 2009